“Characterization of TFPI-2 as a substrate of ADAMTS1 and its relevance during tumor progression”.
Coordinator: Juan Carlos Rodríguez-Manzaneque Escribano.
The catalytic action of ADAMTS1 on TFPI-2 appears to alter the invasive and metastatic capacity of various cell types. Our cellular models included glioblastoma, fibrosarcoma and melanoma, in which it has been observed that the presence of TFPI-2 is relevant for their invasive and metastatic properties. The impact of the vasculogenic mimicry phenomenon in these tumor models is also been evaluated
“Identification of new substrates of the ADAMTS1 metalloprotease by proteomic approaches”.
Coordinator: Juan Carlos Rodríguez-Manzaneque Escribano and Francesc Canals Suris.
In different cellular systems, we are trying to identify extracellular fragments generated by the catalytic activity of ADAMTS1 by advance techniques in proteomics. The relevance of these candidates is being evaluated under various experimental conditions. This project is carried in collaboration with Dr. Canals, leader of the Laboratory of Proteomics.
“Cleavage of proteoglycans by ADAMTS proteases and its implication on angiogenesis and metastatic processes”.
Coordinator: Juan Carlos Rodríguez-Manzaneque Escribano.
Among the targets of ADAMTS proteases, proteoglycans appear to be their most relevant substrates, multi-faceted molecules which role on cancer has been highlighted but still controversial. Our recent findings included transmembrane syndecans as substrates of ADAMTSs with consequences in adhesion and migration. A thoughtful characterization of this proteolytic event in animal models is required.